LAMP (loop-mediated isothermal amplification) detection primer group and kit for tobacco curl stem virus as well as application and method of LAMP detection kit

The invention relates to a tobacco curl stem virus LAMP (loop-mediated isothermal amplification) detection primer group, a kit as well as application and a method thereof, belonging to the technical field of molecular biology. The LAMP detection primer group for the tobacco curl stem virus comprises an outer primer pair TbC-2-F3 and an outer primer pair TbC-2-B3, wherein the nucleotide sequences of the outer primer pair TbC-2-F3 and TbC-2-B3 are shown as SEQ ID NO.1 and SEQ ID NO.2; the nucleotide sequences of the inner primer pair TbC-2-FIP and TbC-2-BIP are as shown in SEQ ID NO.3 and SEQ ID NO.4; the nucleotide sequences of the loop primer pairs TbC-2-LF and TbC-2-LB are as shown in SEQ ID NO. 5 and SEQ ID NO. 6. According to the LAMP detection primer group, the kit and the method for detecting the tobacco stem curl virus, the sensitivity of detecting the tobacco stem curl virus is 10,000 times of that of conventional PCR (Polymerase Chain Reaction) detection. 本发明"烟草曲茎病毒LAMP检测引物组、试剂盒及其应用和方法"属于分子生物学技术领域。所述烟