Primer probe composition, kit and method for detecting canine distemper virus and canine adenovirus II

The invention relates to the technical field of biological detection, in particular to a canine distemper virus and canine adenovirus II type detection primer probe composition, a kit and a detection method. The canine distemper virus and canine adenovirus II type detection primer probe composition provided by the invention comprises a first group of primers and probes which are designed based on a nucleotide sequence as shown in SEQ ID NO.10, and a second group of primers and probes which are designed based on a nucleotide sequence as shown in SEQ ID NO.12; the second group of primers and probes are designed on the basis of a nucleotide sequence as shown in SEQ ID NO.11; and the third group of primers and probes are designed on the basis of the nucleotide sequence as shown in SEQ ID NO.12. The primer probe composition can be used for monitoring the collection, transportation and extraction process of a sample to be detected, and false negative and false positive of a detection result are avoided, so that the