SNP (Single Nucleotide Polymorphism) site primer combination for identifying variety of high-foot chicken and application of SNP site primer combination

The invention discloses a set of SNP site primer combination for high-foot chicken breed identification and application thereof, the primer combination amplifies any three to seven kinds selected from a GJ1 site to a GJ7 site, when the primer combination is used for high-foot chicken breed identification, as long as the genotype of one SNP site does not conform to the corresponding genotype of high-foot chicken, the genotype of the SNP site is not consistent with the corresponding genotype of the high-foot chicken. The individual can be completely excluded from the high-foot chicken; any three SNP site combinations are selected, and the judgment probability of conforming to the genotype of the high-foot chicken can reach 98% or above. The method is easy and convenient to operate and high in accuracy, and a scientific basis is provided for breed conservation and breeding of stock seeds of high-foot chickens. 本发明公开了一套用于高脚鸡品种鉴定的SNP位点引物组合及其应用，所述引物组合扩增选自GJ1位点到GJ7位点中的任意3到7种，利用本发明中的引物组合进行高脚鸡品种鉴定时，只要有一个SNP位点基因型不符合高脚鸡