Culture medium and culture method of peritoneal malignant mesothelioma organoid
The invention discloses a culture medium and a culture method of peritoneal malignant mesothelioma organs. The culture medium comprises an initial culture medium and an amplification culture medium, wherein a formula of a second culture medium comprises Advanced DMEM/F12, 1-2% of P/S double antibodi...
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| creator | CHEN TIANLIANG SHU LIN LI XUANFEI FANG XIAOCHANG FENG MAOHUI |
| description | The invention discloses a culture medium and a culture method of peritoneal malignant mesothelioma organs. The culture medium comprises an initial culture medium and an amplification culture medium, wherein a formula of a second culture medium comprises Advanced DMEM/F12, 1-2% of P/S double antibodies, 1-2% of primocin, 10mM of Hepes, 2-10mM of GlutaMAX, a B27 additive, an N2 additive, 50-125ng/mL of Wnt-3a, 50-125ng/mL of Noggin, 0.5-1.25 [mu] g/mL of R-Spondin1, 100-250ng/mL of FGF10, 35-70ng/mL of EGF, 1-10nM of gasin I, 5.5-10ng/mL of Insulin, 7.5-12.5 [mu The first culture medium is prepared by adding the following compounds with final concentrations on the basis of the second culture medium: 10 [mu] M of Nicotinamide, 10 to 40 [mu] M of Y-27632 and 1.75 to 3.25 [mu] M of CHIR999021. The method is high in induction rate.
本发明公开了一种腹膜恶性间皮瘤类器官的培养基和培养方法，包括初始培养基和扩增培养基：所述第二培养基的配方为：Advanced DMEM/F12，1-2％P/S双抗，1-2％primocin，10mM Hepes，2-10mM GlutaMAX，B27添加剂，N2添加剂，50-125ng/mL Wnt-3a，50-125ng/mL Noggin，0.5-1.25μg/mL |
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本发明公开了一种腹膜恶性间皮瘤类器官的培养基和培养方法，包括初始培养基和扩增培养基：所述第二培养基的配方为：Advanced DMEM/F12，1-2％P/S双抗，1-2％primocin，10mM Hepes，2-10mM GlutaMAX，B27添加剂，N2添加剂，50-125ng/mL Wnt-3a，50-125ng/mL Noggin，0.5-1.25μg/mL</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20231027&DB=EPODOC&CC=CN&NR=116948973A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,309,782,887,25571,76555</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20231027&DB=EPODOC&CC=CN&NR=116948973A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>CHEN TIANLIANG</creatorcontrib><creatorcontrib>SHU LIN</creatorcontrib><creatorcontrib>LI XUANFEI</creatorcontrib><creatorcontrib>FANG XIAOCHANG</creatorcontrib><creatorcontrib>FENG MAOHUI</creatorcontrib><title>Culture medium and culture method of peritoneal malignant mesothelioma organoid</title><description>The invention discloses a culture medium and a culture method of peritoneal malignant mesothelioma organs. The culture medium comprises an initial culture medium and an amplification culture medium, wherein a formula of a second culture medium comprises Advanced DMEM/F12, 1-2% of P/S double antibodies, 1-2% of primocin, 10mM of Hepes, 2-10mM of GlutaMAX, a B27 additive, an N2 additive, 50-125ng/mL of Wnt-3a, 50-125ng/mL of Noggin, 0.5-1.25 [mu] g/mL of R-Spondin1, 100-250ng/mL of FGF10, 35-70ng/mL of EGF, 1-10nM of gasin I, 5.5-10ng/mL of Insulin, 7.5-12.5 [mu The first culture medium is prepared by adding the following compounds with final concentrations on the basis of the second culture medium: 10 [mu] M of Nicotinamide, 10 to 40 [mu] M of Y-27632 and 1.75 to 3.25 [mu] M of CHIR999021. The method is high in induction rate.
本发明公开了一种腹膜恶性间皮瘤类器官的培养基和培养方法，包括初始培养基和扩增培养基：所述第二培养基的配方为：Advanced DMEM/F12，1-2％P/S双抗，1-2％primocin，10mM Hepes，2-10mM GlutaMAX，B27添加剂，N2添加剂，50-125ng/mL Wnt-3a，50-125ng/mL Noggin，0.5-1.25μg/mL</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNirEKwjAQQLM4iPoP5wc4lIrasQTFSRf3cjTXNpDcheTy_zqIs9OD997aPG0NWjNBJOdrBGQH40_pIg5kgkTZqzBhgIjBz4ysn1xEFwpeIoLkGVm825rVhKHQ7suN2d-uL3s_UJKBSsKRmHSwj6Y5dcdLd2779p_nDaFxN7A</recordid><startdate>20231027</startdate><enddate>20231027</enddate><creator>CHEN TIANLIANG</creator><creator>SHU LIN</creator><creator>LI XUANFEI</creator><creator>FANG XIAOCHANG</creator><creator>FENG MAOHUI</creator><scope>EVB</scope></search><sort><creationdate>20231027</creationdate><title>Culture medium and culture method of peritoneal malignant mesothelioma organoid</title><author>CHEN TIANLIANG ; SHU LIN ; LI XUANFEI ; FANG XIAOCHANG ; FENG MAOHUI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN116948973A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>CHEN TIANLIANG</creatorcontrib><creatorcontrib>SHU LIN</creatorcontrib><creatorcontrib>LI XUANFEI</creatorcontrib><creatorcontrib>FANG XIAOCHANG</creatorcontrib><creatorcontrib>FENG MAOHUI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>CHEN TIANLIANG</au><au>SHU LIN</au><au>LI XUANFEI</au><au>FANG XIAOCHANG</au><au>FENG MAOHUI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Culture medium and culture method of peritoneal malignant mesothelioma organoid</title><date>2023-10-27</date><risdate>2023</risdate><abstract>The invention discloses a culture medium and a culture method of peritoneal malignant mesothelioma organs. The culture medium comprises an initial culture medium and an amplification culture medium, wherein a formula of a second culture medium comprises Advanced DMEM/F12, 1-2% of P/S double antibodies, 1-2% of primocin, 10mM of Hepes, 2-10mM of GlutaMAX, a B27 additive, an N2 additive, 50-125ng/mL of Wnt-3a, 50-125ng/mL of Noggin, 0.5-1.25 [mu] g/mL of R-Spondin1, 100-250ng/mL of FGF10, 35-70ng/mL of EGF, 1-10nM of gasin I, 5.5-10ng/mL of Insulin, 7.5-12.5 [mu The first culture medium is prepared by adding the following compounds with final concentrations on the basis of the second culture medium: 10 [mu] M of Nicotinamide, 10 to 40 [mu] M of Y-27632 and 1.75 to 3.25 [mu] M of CHIR999021. The method is high in induction rate.
本发明公开了一种腹膜恶性间皮瘤类器官的培养基和培养方法，包括初始培养基和扩增培养基：所述第二培养基的配方为：Advanced DMEM/F12，1-2％P/S双抗，1-2％primocin，10mM Hepes，2-10mM GlutaMAX，B27添加剂，N2添加剂，50-125ng/mL Wnt-3a，50-125ng/mL Noggin，0.5-1.25μg/mL</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Culture medium and culture method of peritoneal malignant mesothelioma organoid |
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