Culture medium and culture method of peritoneal malignant mesothelioma organoid

The invention discloses a culture medium and a culture method of peritoneal malignant mesothelioma organs. The culture medium comprises an initial culture medium and an amplification culture medium, wherein a formula of a second culture medium comprises Advanced DMEM/F12, 1-2% of P/S double antibodies, 1-2% of primocin, 10mM of Hepes, 2-10mM of GlutaMAX, a B27 additive, an N2 additive, 50-125ng/mL of Wnt-3a, 50-125ng/mL of Noggin, 0.5-1.25 [mu] g/mL of R-Spondin1, 100-250ng/mL of FGF10, 35-70ng/mL of EGF, 1-10nM of gasin I, 5.5-10ng/mL of Insulin, 7.5-12.5 [mu The first culture medium is prepared by adding the following compounds with final concentrations on the basis of the second culture medium: 10 [mu] M of Nicotinamide, 10 to 40 [mu] M of Y-27632 and 1.75 to 3.25 [mu] M of CHIR999021. The method is high in induction rate. 本发明公开了一种腹膜恶性间皮瘤类器官的培养基和培养方法，包括初始培养基和扩增培养基：所述第二培养基的配方为：Advanced DMEM/F12，1-2％P/S双抗，1-2％primocin，10mM Hepes，2-10mM GlutaMAX，B27添加剂，N2添加剂，50-125ng/mL Wnt-3a，50-125ng/mL Noggin，0.5-1.25μg/mL