MMP8 detection method based on CRISPR/Cas12 and nano immunoadsorption method

The invention belongs to the technical field of biological detection, and particularly discloses an MMP8 detection method based on CRISPR/Cas12 and a nano immunoadsorption method, an analyte is recognized and separated through a magnetic bead probe and a gold nanoprobe, an analyte signal is converted and amplified into a nucleic acid signal, a large number of nucleic acid activation chains are used for activating the trans-cleavage activity of CRISPR/Cas12, and the MMP8 detection method based on CRISPR/Cas12 is obtained. Nucleic acid amplification methods such as PCR (Polymerase Chain Reaction) and the like are avoided; and double amplification of signals is realized. Compared with a traditional Elisa method, the method has the advantages that the operation is simpler, the sensitivity is higher, and the specificity is stronger; the detection limit of the MMP8 protein standard substance is 100 fg/ml, a linear relation is formed in the range of 0-100 ng/ml, quantitative analysis can be achieved, and the MMP8 pr