Application of micromolecular heat shock protein IbpA in improving environmental tolerance of host bacteria

The invention provides an application of a micromolecular heat shock protein IbpA in improvement of environmental tolerance of host bacteria, and belongs to the technical field of genetic engineering, which is characterized in that a micromolecular heat shock protein coding gene ibpA in rhodobacter...

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Bibliographische Detailangaben
Hauptverfasser: LIU XINLI, YANG FEIYUE, DONG DIE, QI ZHENGLIANG, YE YIKE, HAN YUJIE
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides an application of a micromolecular heat shock protein IbpA in improvement of environmental tolerance of host bacteria, and belongs to the technical field of genetic engineering, which is characterized in that a micromolecular heat shock protein coding gene ibpA in rhodobacter sphaeroides ATCC 17023 is cloned and an expression vector is constructed, and the expression vector is transferred into the host bacteria, so that the host bacteria overexpresses the micromolecular heat shock protein IbpA, and the environmental tolerance of the host bacteria is improved. The nucleotide sequence of the micromolecular heat shock protein coding gene ibpA is as shown in SEQ ID NO. 1. By overexpressing the micromolecular heat shock protein IbpA in the host bacteria, the resistance of the obtained recombinant bacteria under different stress conditions is greatly increased, and the viability of the host bacteria under various stress conditions can be improved. 本发明提供了一种小分子热休克蛋白IbpA在提高宿主菌环境耐受性中的应用,属于基因工程技术领