Method for expressing and purifying drosophila melanogaster RDGC/CaM protein compound

The invention discloses a method for co-expressing and purifying a drosophila retina denatured protein RDGC and calmodulin CaM protein compound by using an SF9 insect cell expression system. According to the method, an insect cell expression purification system is adopted, a Bac-to-Bac baculovirus e...

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Bibliographische Detailangaben
Hauptverfasser: LIU WEI, LIU JIA, JIANG RITING, CHEN QIANGOU, LIU YUYANG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a method for co-expressing and purifying a drosophila retina denatured protein RDGC and calmodulin CaM protein compound by using an SF9 insect cell expression system. According to the method, an insect cell expression purification system is adopted, a Bac-to-Bac baculovirus expression system is adopted, SF9 insect cells are infected through a transfection reagent, then the insect cells are infected in a co-expression mode, and a large number of target protein RDGC/CaM compounds are obtained. And finally, purifying the fruit fly retinal denatured protein RDGC and calmodulin CaM compound in vitro through a Strep-tag affinity chromatography and gel filtration chromatography method. The method for co-expressing the drosophila melanogaster CaM protein and the drosophila melanogaster RDGC protein through an SF9 insect cell expression system and purifying the compound of the drosophila melanogaster CaM protein and the drosophila melanogaster RDGC/CaM protein has important scientific significa