Domestication method of CHO-K1

The invention belongs to the technical field of biology, and particularly relates to a domestication method of CHO-K1. The conventional domestication method comprises direct domestication and gradient domestication. In a direct domestication process, a cell growth environment is mutated within a short time, and the problems of cell agglomeration, low motility rate and the like are easily caused, and in addition, a direct domestication method needs a large number of initial cells, has higher requirements on a culture field and equipment, and is high in operation difficulty, large in investment and high in failure rate; a gradient domestication method needs to gradually reduce the serum content in a culture medium, the process is tedious, and the time period is long. The'serum-free-serum alternate domestication method 'adopted by the invention does not need a large number of initial cells, has low requirements on culture sites and equipment, does not need gradient reduction of serum, does not need a specific co