Digital nucleic acid detection micro-fluidic chip based on flow fluorescence

The invention discloses a digital nucleic acid detection micro-fluidic chip based on flow fluorescence, and belongs to the field of medical instruments, the digital nucleic acid detection micro-fluidic chip is sequentially provided with a digitization area, an amplification area and a detection area...

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Bibliographische Detailangaben
Hauptverfasser: FANG HAO, LUO GANGYIN, WANG BIDOU, WANG JINXIAN, YANG TIANHANG, ZHU CHUNSEN, WANG ZHENYA, CHEN TINGYI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a digital nucleic acid detection micro-fluidic chip based on flow fluorescence, and belongs to the field of medical instruments, the digital nucleic acid detection micro-fluidic chip is sequentially provided with a digitization area, an amplification area and a detection area which are communicated with one another, the digitization area is provided with a plurality of liquid inlets, a micro-droplet generation structure and a mixing flow channel, and the micro-droplet generation structure comprises a plurality of liquid inlet flow channels; each liquid inlet flow channel is communicated with one liquid inlet, the liquid inlet flow channels intersect at one point to enable liquid in the liquid inlet flow channels to collide to generate micro-droplets, the micro-droplet generation structure is communicated with the amplification flow channel through the mixing flow channel, the amplification flow channel is communicated with the detection area, and the micro-droplets generated by the mic