Method for preparing alfalfa mosaic virus-like particles by using plant expression system and application of alfalfa mosaic virus-like particles

The present invention relates to a technology for preparing and producing an Alfafa mosaic virus (AMV) in the form of virus-like particles (VLP), the AMV being isolated from a plant body transformed with a recombinant vector for expression of the plant body targeted by chloroplast, and provides a recombinant vector comprising a polynucleotide, and a method for preparing and producing the Afafa mosaic virus (AMV) in the form of the VLP, the recombinant vector comprising the polynucleotide, and the method for preparing and producing the Afafa mosaic virus (AMV), the Afafa mosaic virus (AMV), the Afafa mosaic virus (AMV), and the method for preparing and producing the Afafa mosaic virus (AMV). The polynucleotide encodes a recombinant protein fused by a chloroplast targeted protein and an AMV capsid protein. Also provided are a transformed plant body transformed by the recombinant vector, a method for separating and purifying a target protein from the transformed plant body, a method for producing virus-like part