Method for improving stability of high-temperature alkaline protease AprThc and mutant

The invention relates to the field of gene engineering, in particular to a method for improving the stability of high-temperature alkaline protease AprThc and a mutant. According to the application, the alkaline protease AprThc is subjected to L362I, K211G and/or R173G mutation. The high-temperature alkaline protease AprThc is subjected to molecular improvement, so that the thermal stability and the pH stability of the high-temperature alkaline protease AprThc are improved, the method is of great significance in improving the comprehensive performance of the protease and reducing the use cost of the protease, and an effective technical method is provided for property improvement of the protease. 本发明涉及基因工程领域，具体涉及一种改良高温碱性蛋白酶AprThc的稳定性的方法及突变体。本申请对碱性蛋白酶AprThc进行L362I、K211G和/或R173G突变。本申请对高温碱性蛋白酶AprThc进行分子改良，以提升其热稳定性和pH稳定性，对于提升蛋白酶的综合性能，降低蛋白酶的使用成本具有重要意义，为蛋白酶的性质改良提供了有效的技术方法。