Method for synthesizing cucurbitadienol by using cucurbitadienol synthase and application of cucurbitadienol synthase

The invention discloses a method for synthesizing cucurbitadienol by using cucurbitadienol synthase. A cucurbitadienol synthase gene HcOSC6 and a tHMGR gene of avena rough are simultaneously transferred into tobacco to obtain transgenic tobacco for synthesizing cucurbitadienol. The method has the advantages that the obtained transgenic plant can be used for synthesizing cucurbitadienol, so that the cucurbitacine key intermediate can be obtained more efficiently and conveniently. The method is simple, easy to operate and suitable for large-scale production and market popularization and application, and a new way is provided for obtaining the cucurbitacine key intermediate. 本发明公开了一种采用葫芦二烯醇合酶合成葫芦二烯醇的方法，将葫芦二烯醇合酶基因HcOSC6和糙伏毛燕麦的tHMGR基因同时转入烟草中获得合成葫芦二烯醇的转基因烟草。本发明的优点在于：获得的转基因植株能够合成葫芦二烯醇，使得葫芦素关键中间体的获取更加高效便捷。本发明方法简单，易操作，适于规模化生产和市场推广应用，为获取葫芦素关键中间体提供了新途径。