Methods and compositions for determining sensitivity to checkpoint inhibitor treatment

Provided are methods of detecting one or more biomarkers (e.g., cancer) in immune cells of a patient or subject having a condition (e.g., cancer) before and after exposure of tumor cells to an alternating electric field. The present invention relates to methods and kits for treating a subject with a checkpoint inhibitor by using the expression levels of T-cytokines, cytotoxic genes, modulators of immune cell function, immature immune cell markers, modulating T-cytokines and immunosuppressive receptors). Kits comprising nucleic acid probes for detecting the one or more biomarkers are also provided. 提供了通过在肿瘤细胞暴露于交变电场之前和之后检测患有病况(例如，癌症)的患者或受试者的免疫细胞中一种或多种生物标志物(例如，细胞因子和细胞毒性基因、免疫细胞功能调节剂、幼稚免疫细胞标志物、调节T细胞因子和免疫抑制受体)的表达水平来用检查点抑制剂治疗受试者的方法和试剂盒。还提供了包含用于检测所述一种或多种生物标志物的核酸探针的试剂盒。