Method and kit for detecting content of furosine in dairy product

The invention discloses a method and a kit for detecting the content of furosine in a dairy product, and the method for detecting the content of furosine in the dairy product comprises the following steps: hydrolyzing a dairy product sample by using an inorganic acid solution to obtain a hydrolysis...

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Hauptverfasser: LING YUN, YAO GUIHONG, ZHANG YUJIA, ZHANG FENG
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention discloses a method and a kit for detecting the content of furosine in a dairy product, and the method for detecting the content of furosine in the dairy product comprises the following steps: hydrolyzing a dairy product sample by using an inorganic acid solution to obtain a hydrolysis stock solution, and diluting the hydrolysis stock solution to obtain a hydrolysate; detecting the protein content in the hydrolysis stock solution to obtain the protein concentration of the hydrolysis stock solution; furosine standard working solutions with at least three concentrations are provided, the working solutions are mixed solutions of furosine and hydrochloric acid, and the concentration of hydrochloric acid is 0.8-1.5 times of the concentration of inorganic acid in the hydrolysate; and detecting the hydrolysate and the furosine standard working solution by using ultra-high performance liquid chromatography-high resolution time-of-flight mass spectrometry to obtain the content of furosine in the dairy pro