Construction method of collagen-targeted stem cell source exosome

The invention discloses a construction method of a collagen-targeted stem cell source exosome, and relates to the technical field of biomedicine, the method comprises the following steps: S1, constructing TDSCs capable of stably transfecting pCDH-CMV-Puro-CD63-CBD: S1-1, extracting TDSCs and BMSCs o...

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Bibliographische Detailangaben
Hauptverfasser:	QIN TINGWU, LI XUAN, ZHANG YANJING, CUI JING
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DISINFECTION, STERILISATION, OR DEODORISATION OF AIR ENZYMOLOGY HUMAN NECESSITIES HYGIENE MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES MEDICAL OR VETERINARY SCIENCE METALLURGY METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a construction method of a collagen-targeted stem cell source exosome, and relates to the technical field of biomedicine, the method comprises the following steps: S1, constructing TDSCs capable of stably transfecting pCDH-CMV-Puro-CD63-CBD: S1-1, extracting TDSCs and BMSCs of a rat; s1-2, a carrier pCDH-CMV-Puro-CD63-CBD is designed and constructed, and the carrier pCDH-CMV-Puro-CD63-CBD is S1-3, packaging the lentiviral vector and measuring the titer of the lentiviral vector; s1-4, a stable strain of the TDSCs transfected with the pCDH-CMV-Puro-CD63-CBD is constructed, and the stable strain of the TDSCs transfected with the pCDH- And S2, extracting the TDSCs source exosome of the transfected pCDH-CMV-Puro-CD63-CBD, and carrying out identification on the TDSCs source exosome. The collagen-targeted stem cell source exosome constructed by the method disclosed by the invention can be applied to loading the collagen-targeted stem cell source exosome on a collagen-containing decellularized