Double-chain nucleic acid molecular weight determination method

The invention discloses a double-stranded nucleic acid molecular weight determination method which comprises the following steps: preparing a reference substance solution and a to-be-detected sample solution, analyzing a reference substance and a to-be-detected sample under the same chromatographic condition by using gel chromatography, drawing a standard curve according to the chromatographic result of the reference substance, and calculating the molecular weight of the to-be-detected sample according to the standard curve. The step of preparing the reference substance solution and the to-be-detected sample solution is the step of preparing a polyinosinic-cytidylic acid test solution and a sample solution for comparison with the polyinosinic-cytidylic acid test solution. The method is small in error; detection results can be unified, the proportion of polymer components is convenient to judge, and the detection method is simple in operation steps, few in influence factors, high in reproducibility, short in d