Method for determining optimal intervention concentration of kidney-tonifying arthralgia-dredging prescription on human cartilage cells

The invention discloses a method for determining the optimal intervention concentration of a kidney-tonifying arthralgia-dredging prescription on human cartilage cells, which comprises the following steps: culturing the cartilage cells: inoculating the human cartilage cells into a culture dish at a proper density; the preparation method comprises the following steps: decocting with water, filtering, concentrating, sterilizing and the like. Intervention concentration selection is carried out, and drugs with different concentrations are prepared; cell experiment: adding the kidney-tonifying arthralgia-dredging formulas with different concentrations into the culture medium so as to achieve the required final concentration; cell evaluation: detecting the intervention effect of the kidney tonifying and arthralgia dredging prescriptions with different concentrations on the human cartilage cells; and data analysis: analyzing changes of survival, proliferation, differentiation or other related indexes of the cells. A