Disease-resistant and quality-improved pig breeding method capable of simultaneously knocking out CD163, pAPN and MSTN genes

The invention provides a disease-resistant and quality-improved pig breeding method with CD163, pAPN and MSTN genes knocked out simultaneously, and belongs to the technical field of animal breeding. According to the invention, pig CD163, pAPN and MSTN genes are knocked out at fixed points through a gene editing technology, and in view of the fact that offspring of an introduced commercial pig breed after MSTN gene editing is easy to have a malformation phenotype of weakness of hind limbs, the MSTN gene editing is introduced into the commercial pig breed to hybridize with an MSTN gene editing local pig; the obtained MSTN gene edited binary hybrid pig highlights the variety characteristics of a lean meat type commercial pig breed and a fat type local pig breed, retains the dominant phenotype of MSTN gene editing on the lean meat rate and meat quality improvement of the pig breed, and hybridizes the MSTN gene edited binary hybrid pig with a CD163 and pAPN double-gene edited pig to realize rapid aggregation of th