Application of translation regulatory element in promoting expression of recombinant protein, expression cassette, vector, expression system and kit

The invention belongs to the technical field of gene engineering, and particularly relates to application of a translation enhancing element to promotion of recombinant protein expression, an expression box, a vector, an expression system and a kit. The construction method comprises the following steps: performing structure-function relationship analysis on an insertion sequence of a human genome translation regulatory element 5 '-UTR, screening and designing a nucleotide sequence as shown in SEQ ID NO: 1 as a translation regulatory element, closely connecting the translation regulatory element to the upstream of a target protein coding gene, constructing a recombinant protein expression vector, transfecting the recombinant protein expression vector to mammalian cells, and performing recombinant protein expression. The recombinant expression quantity, especially the transient expression quantity, is improved. Experiments prove that compared with an expression system lacking a translation regulatory element, t