Method for promoting platelet differentiation in vitro

The invention relates to the technical field of medicines, in particular to a method for promoting platelet differentiation in vitro, which is characterized in that human plasma (hp) or human hyperlipidemia plasma (hlp) is added into a culture medium in the process of generating platelets from human induced stem cells (hiPSC) in vitro. Results of the invention show that in-vivo platelet counting is related to hyperlipidemia, and human plasma (hp) mainly promotes differentiation of CD34 + hematopoietic cells into megakaryocyte-progenitor cells (MKPs) in 8-14 days in a platelet generation process by in-vitro hiPSCs. Fatty acid (FA) is an active ingredient for promoting hipsc-derived megakaryocyte (MK) and platelet generation, and on the fourteenth day, FA activates MK and platelet generation in MKPs through a JAK2/STAT/AKT pathway. The invention expands the platelet differentiation theory and provides a method for promoting platelet differentiation in vitro. 本发明涉及医药技术领域，具体是一种体外促进血小板分化的方法，是在体外人诱导干细胞(hiPSC)生成血小板过