EcN engineering bacterium for targeted recognition and cleavage of multi-drug-resistance genes as well as construction method and application of EcN engineering bacterium

The invention discloses an engineering bacterium for targeted recognition and cleavage of multi-drug-resistant genes and a construction method and application thereof, the engineering bacterium takes probiotics EcN as a chassis bacterium, and is integrated with an I-E type CRISPR-Cas system: a gene for coding Cas3 protein and a cas operon for coding a Casade protein complex are expressed by an arabinose-induced promoter PBAD on a genome; cRISPR (CRMNT) for targeted recognition of multi-drug resistance genes is expressed on a plasmid through a promoter PJ23100; the EcN engineering bacterium constructed by the invention can simultaneously reduce a plurality of drug-resistant genes in a targeting manner, further improves the safety and operability of the EcN engineering bacterium as a chassis bacterium, not only is beneficial to improving the biological safety of viable bacteria drugs, but also can be used as an excellent strain for producing various products (such as vitamins, proteins and amino acids) in produ