Long-term in-vitro preservation method for endangered dendrobium germplasm

The invention discloses a long-term in-vitro preservation method of endangered dendrobium germplasm, which comprises the following two steps: the first stage is a growth inhibition stage for long-term preservation of sterile dendrobium seeds by using a growth inhibition culture medium, and the second stage is a growth recovery stage for seedling induction culture by using a growth recovery culture medium; the formula of the growth inhibition culture medium comprises 40-80g/L of oat and 6g/L of agar; the formula of the recovery growth culture medium comprises 1/2MS, 1.0 g/L of peptone, 1.0 g/L of activated carbon, 25 g/L of cane sugar, 6.0 g/L of agar, 50 g/L of potatoes, 80 g/L of bananas and 15 g/L of apples. According to the method, organic additives are utilized, a proper culture medium is prepared, the PH value is adjusted, the germplasm germination rate is high, differentiation is avoided, the mutation rate is low, the transfer period is long in the growth inhibition period, the differentiation rate is h