Method for producing biochemical products using enzyme genes derived from brevundimonas strains and compositions prepared thereby

Disclosed is a crtW gene from a brevundimonas strain encoding a novel ketoenzyme for carotenoid synthesis. Exemplary synthetic operands containing additional related carotenoid gene sequences are also provided, wherein expression of the synthetic operands is used to produce ketocarotenoids. Suitable DNA expression constructs derived from these sequences are inserted into expression hosts for expression. The expression products are ketoenzymes that can be used to convert beta-carotene to canthaxanthin and astaxanthin. 公开了来自短波单胞菌属菌株的crtW基因，其编码用于类胡萝卜素合成的新型酮酶。还提供了含有额外的相关类胡萝卜素基因序列的示例性合成操纵子，其中合成操纵子的表达用于产生酮基类胡萝卜素。将源自这些序列的合适DNA表达构建体插入表达宿主中用于表达。所述表达产物是可用于将β-胡萝卜素转化为角黄素和虾青素的酮酶。