Method for controlled production of monodisperse microbubbles

The present invention relates to a process for preparing a hydrated phospholipid solvent mixture, the process comprising:-dissolving a first phospholipid in an organic solvent at a temperature above the phase transition temperature of the phospholipid to form a dissolved phospholipid solvent mixture; dissolving a second phospholipid in the dissolved phospholipid solvent mixture at a temperature above the phase transition temperature of the phospholipid to form a dissolved phospholipid solvent mixture; adding an aqueous phosphate buffer to the dissolved phospholipid solvent mixture to form a buffered phospholipid solvent mixture; and-agitating the buffered phospholipid solvent mixture to form a hydrated phospholipid solvent mixture. 本发明涉及一种用于制备水合磷脂溶剂混合物的方法，该方法包括：-在高于磷脂的相变温度的温度下将第一磷脂溶解在有机溶剂中，以形成溶解的磷脂溶剂混合物；-在高于磷脂的该相变温度的温度下将第二磷脂溶解在该溶解的磷脂溶剂混合物中，以形成溶解的磷脂溶剂混合物；-将水性磷酸盐缓冲液加入到该溶解的磷脂溶剂混合物中，以形成缓冲磷脂溶剂混合物；以及-搅拌该缓冲磷脂溶剂混合物，以形成水合磷脂溶剂混合物。