Quantitative fingerprint spectrum construction method for medicinal components of Weishenchun capsule and application of quantitative fingerprint spectrum construction method

The invention belongs to the technical field of traditional Chinese medicine detection, and provides a construction method and application of a quantitative fingerprint spectrum of medicinal components of Weishenchun capsules. The construction method comprises a chromatographic analysis step, a chro...

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Hauptverfasser: CHEN WEI, ZHONG YI, ZHANG WEI, LU XIAOJING, JIN QIANG, CHENG XINMEI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the technical field of traditional Chinese medicine detection, and provides a construction method and application of a quantitative fingerprint spectrum of medicinal components of Weishenchun capsules. The construction method comprises a chromatographic analysis step, a chromatographic analysis mobile phase A is a 0.5% formic acid aqueous solution, and a mobile phase B is acetonitrile; according to the linear gradient elution procedure, the volume fraction of the mobile phase B is 5%-17% in 0-12 min, the volume fraction of the mobile phase B is 17%-19% in 12-19 min, the volume fraction of the mobile phase B is 17%-23% in 19-40 min, the volume fraction of the mobile phase B is 23%-38% in 40-65 min, the volume fraction of the mobile phase B is 38%-70% in 65-75 min, and the volume fraction of the mobile phase B is 70%-75% in 75-80 min; the chromatographic analysis is used for determining the content of one or more of the 19 components. 本发明属于中药检测技术领域,本发明提供了一种胃复春胶囊药效成分定量指纹图谱的构建方法及其应用。所述的构建