Anti-avian encephalomyelitis virus monoclonal antibody hybridoma cell strain, monoclonal antibody, reagent or kit and application thereof

The invention belongs to the technical field of biotechnology detection, and particularly relates to an anti-avian encephalomyelitis virus monoclonal antibody hybridoma cell strain, a monoclonal antibody, a reagent or a kit and application of the anti-avian encephalomyelitis virus monoclonal antibody hybridoma cell strain. The invention provides a monoclonal antibody hybridoma cell strain AEMab-VP1-25 for resisting avian encephalomyelitis virus, wherein the preservation number of the hybridoma cell strain AEMab-VP1-25 is CGMCC (China General Microbiological Culture Collection Center) NO: 45613. The indirect immunofluorescence kit prepared from the monoclonal antibody secreted by the monoclonal antibody hybridoma cell strain for resisting the avian encephalomyelitis virus has good specificity. The kit not only can be used for detecting exogenous viruses of the AEV in avian virus live vaccines, but also can be used for clinical detection and epidemiological investigation of the AEV. 本发明属于生物技术检测技术领域，具体涉及一种抗禽脑脊髓炎