Recombinant single-domain antibody and construction method thereof

The invention belongs to the field of biomedicine, and relates to a recombinant single-domain antibody capable of being efficiently expressed, the amino acid sequence of the recombinant single-domain antibody is an amino acid sequence formed by adding a signal peptide amino acid sequence to the N terminal of the single-domain antibody and setting a termination codon amino acid sequence to the C terminal of the single-domain antibody, the single-domain antibody is selected from one or more of VHH1, VHH2, VHH3, VHH4, VHH5 and VHH6, and the signal sequence is selected from one or more of Azu, A2, HC, Section, IFN alpha 2 and IL-2. Through codon optimization and addition of the signal peptide, the obtained single-domain antibody composition not only has high biological activity, but also has relatively high protein yield, and can meet the requirements of the application of the single-domain antibody in the early stage and the middle stage. 本发明属于生物医学领域，涉及一种可高效表达的重组单域抗体，所述重组单域抗体的氨基酸序列为在单域抗体的N端添加信号肽氨基酸序列，在单域抗体的C端设置终