Efficient extraction method and application of flavone in peanut shells
The invention discloses an efficient extraction method and application of flavone in peanut shells, the peanut shells are cleaned, aired and dried, and then crushed and sieved to obtain peanut shell powder; weighing 50 mg of peanut shell powder, adding the peanut shell powder into a 1-5 mL centrifug...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses an efficient extraction method and application of flavone in peanut shells, the peanut shells are cleaned, aired and dried, and then crushed and sieved to obtain peanut shell powder; weighing 50 mg of peanut shell powder, adding the peanut shell powder into a 1-5 mL centrifugal tube, adding 1 mL of the eutectic solution, carrying out vortex uniform mixing, carrying out ultrasonic-assisted extraction at 30-60 DEG C for 30-60 min, carrying out centrifugation at a rotating speed of 12000 rpm/min for 10 min, taking a supernatant, and diluting the supernatant with chromatographically pure methanol in an amount which is 5 times that of the supernatant to obtain the peanut total flavonoids, according to the method, the extraction rate of flavone in the peanut shells can be increased.
本发明公开了一种花生壳中黄酮的高效提取方法及应用,将花生壳洗净,晾晒烘干后,粉碎过筛,得花生壳粉末;称量花生壳粉末50 mg加入到1~5 mL离心管中,加入低共熔溶液1 mL,涡旋混匀,在30~60℃下超声辅助提取30~60 min,在转速为12000 rpm/min条件下离心10 min,取上清液,用上清液5倍量色谱纯甲醇稀释,即得花生总黄酮;本申请可以提高花生壳中黄酮的提取率。 |
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