Quantitative detection primer probe composition based on microdroplet type digital PCR (Polymerase Chain Reaction) and application of quantitative detection primer probe composition

The invention discloses a primer probe composition for quantitative detection of a daisfanda virus based on micro-droplet digital PCR (Polymerase Chain Reaction) and application of the primer probe composition. The method is good in universality and suitable for merging the DBV strain sequences which are globally popular at present, and the upstream primer, the downstream primer and the probe. The monoclonal antibody disclosed by the invention is good in specificity, and has no cross reaction with the arthroviruses such as ZIKA, CHIKV, DENV, JEV and the like. The sensitivity is high, and the detection limit can reach 6 copies/reaction in a 20 microliter system. According to the method, absolute quantification of the virus load can be realized, a standard curve does not need to be set, the copy number of the Databan virus in the sample can be directly judged according to a detection result, and the operation steps are greatly simplified. 本发明公开了一种基于微滴式数字PCR的大别班达病毒定量检测引物探针组合物及其应用。本发明通用性好，适用于目前全球流行的DBV毒株序列，上下游引物及