Molecular marker for detecting rice cold-resistant gene CTB4a and application

The invention relates to a molecular marker for detecting a rice cold-resistant gene CTB4a and application, and the molecular marker is a specific molecular marker and is located at the SNP-1930 position of a CTB4a coding gene. The base polymorphism of the SNP site of the specific molecular marker i...

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Bibliographische Detailangaben
Hauptverfasser:	SUN YIDING, TANG CUIFENG, A XINXIANG, DAI LUYUAN, MA JIQIONG, YANG YI, XU MINGHUI, ZHANG FEIFEI, YANG YAYUN, DONG CHAO, WANG BIN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a molecular marker for detecting a rice cold-resistant gene CTB4a and application, and the molecular marker is a specific molecular marker and is located at the SNP-1930 position of a CTB4a coding gene. The base polymorphism of the SNP site of the specific molecular marker is G/C; if the basic group at the site is G, the rice material to be detected has a cold-resistant gene CTB4a, and if the basic group at the site is C, the rice material to be detected carries a non-cold-resistant gene haplotype ctb4a of the CTB4a. The detection primer combination of the specific molecular marker comprises a primer ctb4a-1930F and a primer ctb4a-1930R, and a restriction enzyme Hind III. The detection primer combination of the specific molecular marker comprises a primer ctb4a-1930F, a primer ctb4a-1930R and a restriction enzyme Hind III. The specific molecular marker disclosed by the invention is applied to the identification of the rice cold-tolerant gene CTB4a. The primer combination is applied to