SNP (Single Nucleotide Polymorphism) site primer combination for identifying breeds of black-bone silky fowl and application of SNP site primer combination

The invention discloses an SNP (Single Nucleotide Polymorphism) site primer combination for identifying breeds of black-bone silky fowl and application of the SNP site primer combination. The primer combination is selected from any 3 to 8 of a WMWG1 site to a WMWG8 site for amplification. When the p...

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Bibliographische Detailangaben
Hauptverfasser:	LUO KEYIN, LIU YIFAN, TU YUNJIE, SHENG ZHONGWEI, ZOU JIANMIN, WANG XIANKE, JU XIAOJUN, JI GAIGE, SHU JINGTING, LI CHUNMIAO, ZHANG MING, WANG GUANJI, SHAN YANJU, XIAO QIN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses an SNP (Single Nucleotide Polymorphism) site primer combination for identifying breeds of black-bone silky fowl and application of the SNP site primer combination. The primer combination is selected from any 3 to 8 of a WMWG1 site to a WMWG8 site for amplification. When the primer combination disclosed by the invention is used for identifying the variety of the UMongolia black-bone chicken, as long as one SNP locus genotype does not conform to the corresponding genotype of the UMongolia black-bone chicken, the individual can be completely excluded from belonging to the UMongolia black-bone chicken; any three SNP site combinations are selected, and the judgment probability of conforming to the genotype of the UMongolia black-bone chicken can reach 97% or above. The identification method is simple and convenient to operate and high in accuracy, can be used for rapidly identifying the UMongolia black-bone chicken variety, and effectively attacks counterfeit and shoddy products of the loca