Detection method of lentivirus integration site and application thereof

The invention discloses a lentivirus integration site detection method and application thereof, and the detection method comprises the following steps: S100, extracting DNA, and breaking; s200, carrying out terminal repair and A addition on the broken DNA, amplifying by adopting a linker primer, and connecting an asymmetric linker; s300, taking the PCR product in the step S200 as a template, adopting a first-round primer, and introducing a blocker for amplification; s400, taking the PCR product in the step S300 as a template, adopting a second round of primer, and introducing a blocker for amplification; meanwhile, a sequencing primer combination sequence of a sequencing platform is introduced; s500, sequencing the PCR product obtained in the step S400 on a machine; s600, filtering the sequencing data; and S700, judging a lentivirus integration event according to the filtered data. On the basis of a linker mediated PCR technology, insertion sites are enriched through two-step PCR, and compared with other libr