Carbonyl reductase, mutant of carbonyl reductase and application of carbonyl reductase in preparation of chiral (R)-8-chloro-6-hydroxy ethyl caprylate

The invention relates to the technical field of gene engineering of enzymes, and provides carbonyl reductase, a mutant thereof and application of the carbonyl reductase in preparation of chiral (R)-8-chloro-6-hydroxy ethyl caprylate in order to solve the problems of slow reaction rate and low production efficiency in preparation of chiral (R)-8-chloro-6-hydroxy ethyl caprylate by a biological catalysis method in the prior art. The amino acid sequence of the carbonyl reductase is as shown in SEQ ID NO.1, the nucleotide sequence of the carbonyl reductase is as shown in SEQ ID NO.2, the amino acid sequence of the mutant is obtained by mutation of the amino acid sequence as shown in SEQ ID NO.1, and mutation comprises at least one of the following mutation sites: 126 site, 129 site and 194 site. The carbonyl reductase and the mutant thereof have good substrate tolerance and catalytic activity, can improve the reaction efficiency, and can be applied to catalysis of asymmetric reduction reaction of prochiral carbon