Preparation method of umbilical cord mesenchymal stem cells

The invention relates to the technical field of cell culture, in particular to a preparation method of umbilical cord mesenchymal stem cells, different culture media and operation methods are adopted in the primary culture process and the subculture process of the umbilical cord mesenchymal stem cells respectively, a digestion method is adopted in the primary culture process, the primary culture time of the umbilical cord mesenchymal stem cells is effectively shortened, and the yield of the umbilical cord mesenchymal stem cells is increased. The verified inoculation density and culture solution replacement time are used in passage, so that the proliferation activity of the umbilical cord mesenchymal stem cells is improved. According to the preparation method disclosed by the invention, the cells can grow from the edge of the tissue within a short time, the number of initial cells is increased, more primary cells can be amplified within the growth time of conventional primary cells, and the proliferation rate