Preparation method of CKLF1 hydrogel

The invention discloses a preparation method of CKLF1 hydrogel. The preparation method specifically comprises the following steps: mixing CKLF1-C19 with purified water to obtain a solution A; adding vitamin A and vitamin E into purified water to obtain a solution B; adding hyaluronic acid into purified water to obtain a solution C; the solution A is added into a mixed solution of the solution B and the solution C, the ratio of the solution A to the solution B to the solution C is 1: 3: 6, and a photo-crosslinking agent i2959 accounting for 0.05%-0.2% of the total mass is added; under ultraviolet light with the light source wavelength of 350-400 nm, the irradiation intensity is 10.0 mW/cm < 2 >, and the crosslinking time is 5-10 min; and after the crosslinking is finished, freezing and drying to obtain the hydrogel containing the CKLF1. According to the invention, all the components in a proper proportion are adopted, so that the mechanical property of the hydrogel is enhanced; the photo-crosslinking agent is