Method for methylated base level detection in nucleic acids

The present invention includes improved methods and compositions for detecting methylation in nucleic acids. In particular, the present disclosure relates to methods of converting 5-hydroxymethylcytosine (5hmC) and/or 5-methylcytosine (5mC) to thymine (T). In addition, the present disclosure also re...

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Bibliographische Detailangaben
Hauptverfasser:	RANIK MARTIN, DE BEER, ANNE, HEINDL DIETER, CRISALLI PETER, PUNKLER JAN-ALBERT, TAING MARVIN, KAXHUR OMER, PUNKLER, DAVID, L, BERGMANN FRANK, CHANG STEPHEN S
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention includes improved methods and compositions for detecting methylation in nucleic acids. In particular, the present disclosure relates to methods of converting 5-hydroxymethylcytosine (5hmC) and/or 5-methylcytosine (5mC) to thymine (T). In addition, the present disclosure also relates to methods of detecting 5hmC and/or 5mC in a sample. 本发明包括用于检测核酸中的甲基化的改进方法和组合物。特别地，本公开涉及将5-羟甲基胞嘧啶(5hmC)和/或5-甲基胞嘧啶(5mC)转化为胸腺嘧啶(T)的方法。此外，本公开还涉及检测样品中的5hmC和/或5mC的方法。