Method for detecting double-copy chromosome linkage carrying of pathogenic gene

The invention relates to a method for detecting double-copy chromosome linkage carrying of a virulence gene, in particular to a method for detecting 2 + 0 carrying of the virulence gene. The method for detecting virulence gene 2 + 0 carrying comprises the following steps: (1) providing genome DNA samples of at least two generations of families including a subject; (2) acquiring long fragment DNA of the target pathogenic gene; (3) sequencing the long fragment DNA by using a long-read-long sequencing platform and constructing haplotypes; and (4) analyzing the family haplotype to obtain whether the pathogenic gene of the subject is carried by 2 + 0 or not. 本申请涉及一种检测致病基因双拷贝染色体连锁携带的方法，尤其涉及一种用于检测致病基因2+0携带的方法。所述用于检测致病基因2+0携带的方法包括以下步骤：（1）提供包括受试者在内的至少两代家系的基因组DNA样本；（2）获取目标致病基因的长片段DNA；（3）利用长读长测序平台对长片段DNA测序并构建单体型；（4）通过家系单体型分析得到受试者致病基因是否为2+0携带。