Construction method of nucleoside-producing engineering bacteria and method for producing nucleoside

The invention provides a construction method of a nucleoside-producing engineering bacterium and a method for producing nucleoside. Glucokinase (coded by a glcK gene) of modified bacillus subtilis or bacillus amyloliquefaciens enhances the activity of the GlcK protein; furthermore, by modifying a glucose/mannose transporter GlcP (encoded by a glcP gene), the activity of the GlcP protein is enhanced, and the guanine nucleoside or hypoxanthine nucleoside can be efficiently and quickly generated by microorganisms, so that an effective means is provided for large-scale nucleoside production, and the application prospect is wide. 本发明提供一种产核苷工程菌的构建方法及生产核苷的方法。经过修饰枯草芽孢杆菌或解淀粉芽孢杆菌的葡萄糖激酶(由glcK基因编码)，使得GlcK蛋白活性增强；进一步地，通过修饰葡萄糖/甘露糖转运蛋白GlcP(由glcP基因编码)，使得GlcP蛋白活性增强，使得微生物能够高效率、快速地生成鸟嘌呤核苷或次黄嘌呤核苷，从而为大规模生产核苷提供有效手段，应用前景广阔。