Kit for detecting alpha-synuclein aggregate

The invention discloses a kit for detecting alpha-synuclein aggregates, and relates to the technical field of biology. The kit comprises an alpha-synuclein monomer, ammonium sulfate and thioflavin T, by changing incubation conditions, the FFs diluted to 10pg/mL can be detected for the first time, 1...

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Hauptverfasser: MAO HENGXU, LI HONGYAN, XU ZHUOFU, LU LIN, WU WENJUN, HUANG XIAOYUN, KUANG YAOYUN, YANG XINLING, YONG YUXUAN, GUO WENYUAN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a kit for detecting alpha-synuclein aggregates, and relates to the technical field of biology. The kit comprises an alpha-synuclein monomer, ammonium sulfate and thioflavin T, by changing incubation conditions, the FFs diluted to 10pg/mL can be detected for the first time, 1 microliter of sample is converted into the total detection amount of 10fg PFFs, and the sensitivity of a single cycle reaches fg level. According to the present invention, the PFFs detection technology has characteristics of high specificity, high sensitivity, rapidness, simpleness, convenience and effectiveness, can replace the brain micro biopsy (invasive), and can be used for the alpha-synuclein aggregate detection of trace body fluids or micro tissues, and the PFFs detection technology has characteristics of high specificity, high sensitivity, rapidness, simpleness, convenience and effectiveness, and can be used for the detection of the alpha-synuclein aggregate of the trace body fluids or the micro tissues, an