Method for detecting rabies vaccine DNA (Deoxyribonucleic Acid) residue by applying digital PCR (Polymerase Chain Reaction)
The invention discloses a method for detecting rabies vaccine DNA (Deoxyribonucleic Acid) residues by applying digital PCR (Polymerase Chain Reaction). The method comprises the following steps: extracting DNA in a human rabies vaccine (Vero cell) stock solution sample; establishing a digital PCR sys...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Patent |
| Sprache: | chi ; eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | SUN JIALING LUO XIAOMEI ZHAO HAIFENG XU HE LI XINXIN ZOU SHU SU ZHE HAN XIAOHONG CHI HUI WANG XUEHAN FANG SHAOPENG WANG LU KANG XINYUAN SHEN YUHUA ZHOU DI CHENG QIYUE GAO YASONG LI BOYUAN HU XUESONG SONG XUE WANG XUE LYU XIN |
| description | The invention discloses a method for detecting rabies vaccine DNA (Deoxyribonucleic Acid) residues by applying digital PCR (Polymerase Chain Reaction). The method comprises the following steps: extracting DNA in a human rabies vaccine (Vero cell) stock solution sample; establishing a digital PCR system; preparing micro-droplets; carrying out digital PCR (Polymerase Chain Reaction) amplification; the Vero cell DNA residual copy number concentration in a human rabies vaccine sample obtained through digital PCR amplification is calculated as a national standard unit value. A primer and a probe are designed according to a GP gene sequence of the rabies virus, reaction conditions and reaction procedures are optimized, and the established digital PCR method is evaluated through specificity and repeatability tests, so that the method for detecting the human rabies vaccine DNA residues by using the digital PCR is established, and the method is good in specificity and repeatability, easy to operate and high in detecti |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN116790822A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN116790822A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN116790822A3</originalsourceid><addsrcrecordid>eNqNjb0KwjAURrs4iPoO100HQSv4M5aquFiKuMtt8qkXYlKSVCy-vAo-gNNZzuF0k9cB8eY0XZwnjQgVxV7JcyUI9GClxII2RUajDdyz9VI52ygDUZQp0WPyCKIbUNUS17Vpv7mWq0Q2VOZHGpXOtHd4DqD8xmLpCP5cnB33k86FTcDgx14y3G1P-X6C2p0RalawiOe8mM0Wy_V0labZ_B_nDa4eRaI</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Method for detecting rabies vaccine DNA (Deoxyribonucleic Acid) residue by applying digital PCR (Polymerase Chain Reaction)</title><source>esp@cenet</source><creator>SUN JIALING ; LUO XIAOMEI ; ZHAO HAIFENG ; XU HE ; LI XINXIN ; ZOU SHU ; SU ZHE ; HAN XIAOHONG ; CHI HUI ; WANG XUEHAN ; FANG SHAOPENG ; WANG LU ; KANG XINYUAN ; SHEN YUHUA ; ZHOU DI ; CHENG QIYUE ; GAO YASONG ; LI BOYUAN ; HU XUESONG ; SONG XUE ; WANG XUE ; LYU XIN</creator><creatorcontrib>SUN JIALING ; LUO XIAOMEI ; ZHAO HAIFENG ; XU HE ; LI XINXIN ; ZOU SHU ; SU ZHE ; HAN XIAOHONG ; CHI HUI ; WANG XUEHAN ; FANG SHAOPENG ; WANG LU ; KANG XINYUAN ; SHEN YUHUA ; ZHOU DI ; CHENG QIYUE ; GAO YASONG ; LI BOYUAN ; HU XUESONG ; SONG XUE ; WANG XUE ; LYU XIN</creatorcontrib><description>The invention discloses a method for detecting rabies vaccine DNA (Deoxyribonucleic Acid) residues by applying digital PCR (Polymerase Chain Reaction). The method comprises the following steps: extracting DNA in a human rabies vaccine (Vero cell) stock solution sample; establishing a digital PCR system; preparing micro-droplets; carrying out digital PCR (Polymerase Chain Reaction) amplification; the Vero cell DNA residual copy number concentration in a human rabies vaccine sample obtained through digital PCR amplification is calculated as a national standard unit value. A primer and a probe are designed according to a GP gene sequence of the rabies virus, reaction conditions and reaction procedures are optimized, and the established digital PCR method is evaluated through specificity and repeatability tests, so that the method for detecting the human rabies vaccine DNA residues by using the digital PCR is established, and the method is good in specificity and repeatability, easy to operate and high in detecti</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROCESSES USING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230922&DB=EPODOC&CC=CN&NR=116790822A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230922&DB=EPODOC&CC=CN&NR=116790822A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>SUN JIALING</creatorcontrib><creatorcontrib>LUO XIAOMEI</creatorcontrib><creatorcontrib>ZHAO HAIFENG</creatorcontrib><creatorcontrib>XU HE</creatorcontrib><creatorcontrib>LI XINXIN</creatorcontrib><creatorcontrib>ZOU SHU</creatorcontrib><creatorcontrib>SU ZHE</creatorcontrib><creatorcontrib>HAN XIAOHONG</creatorcontrib><creatorcontrib>CHI HUI</creatorcontrib><creatorcontrib>WANG XUEHAN</creatorcontrib><creatorcontrib>FANG SHAOPENG</creatorcontrib><creatorcontrib>WANG LU</creatorcontrib><creatorcontrib>KANG XINYUAN</creatorcontrib><creatorcontrib>SHEN YUHUA</creatorcontrib><creatorcontrib>ZHOU DI</creatorcontrib><creatorcontrib>CHENG QIYUE</creatorcontrib><creatorcontrib>GAO YASONG</creatorcontrib><creatorcontrib>LI BOYUAN</creatorcontrib><creatorcontrib>HU XUESONG</creatorcontrib><creatorcontrib>SONG XUE</creatorcontrib><creatorcontrib>WANG XUE</creatorcontrib><creatorcontrib>LYU XIN</creatorcontrib><title>Method for detecting rabies vaccine DNA (Deoxyribonucleic Acid) residue by applying digital PCR (Polymerase Chain Reaction)</title><description>The invention discloses a method for detecting rabies vaccine DNA (Deoxyribonucleic Acid) residues by applying digital PCR (Polymerase Chain Reaction). The method comprises the following steps: extracting DNA in a human rabies vaccine (Vero cell) stock solution sample; establishing a digital PCR system; preparing micro-droplets; carrying out digital PCR (Polymerase Chain Reaction) amplification; the Vero cell DNA residual copy number concentration in a human rabies vaccine sample obtained through digital PCR amplification is calculated as a national standard unit value. A primer and a probe are designed according to a GP gene sequence of the rabies virus, reaction conditions and reaction procedures are optimized, and the established digital PCR method is evaluated through specificity and repeatability tests, so that the method for detecting the human rabies vaccine DNA residues by using the digital PCR is established, and the method is good in specificity and repeatability, easy to operate and high in detecti</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjb0KwjAURrs4iPoO100HQSv4M5aquFiKuMtt8qkXYlKSVCy-vAo-gNNZzuF0k9cB8eY0XZwnjQgVxV7JcyUI9GClxII2RUajDdyz9VI52ygDUZQp0WPyCKIbUNUS17Vpv7mWq0Q2VOZHGpXOtHd4DqD8xmLpCP5cnB33k86FTcDgx14y3G1P-X6C2p0RalawiOe8mM0Wy_V0labZ_B_nDa4eRaI</recordid><startdate>20230922</startdate><enddate>20230922</enddate><creator>SUN JIALING</creator><creator>LUO XIAOMEI</creator><creator>ZHAO HAIFENG</creator><creator>XU HE</creator><creator>LI XINXIN</creator><creator>ZOU SHU</creator><creator>SU ZHE</creator><creator>HAN XIAOHONG</creator><creator>CHI HUI</creator><creator>WANG XUEHAN</creator><creator>FANG SHAOPENG</creator><creator>WANG LU</creator><creator>KANG XINYUAN</creator><creator>SHEN YUHUA</creator><creator>ZHOU DI</creator><creator>CHENG QIYUE</creator><creator>GAO YASONG</creator><creator>LI BOYUAN</creator><creator>HU XUESONG</creator><creator>SONG XUE</creator><creator>WANG XUE</creator><creator>LYU XIN</creator><scope>EVB</scope></search><sort><creationdate>20230922</creationdate><title>Method for detecting rabies vaccine DNA (Deoxyribonucleic Acid) residue by applying digital PCR (Polymerase Chain Reaction)</title><author>SUN JIALING ; LUO XIAOMEI ; ZHAO HAIFENG ; XU HE ; LI XINXIN ; ZOU SHU ; SU ZHE ; HAN XIAOHONG ; CHI HUI ; WANG XUEHAN ; FANG SHAOPENG ; WANG LU ; KANG XINYUAN ; SHEN YUHUA ; ZHOU DI ; CHENG QIYUE ; GAO YASONG ; LI BOYUAN ; HU XUESONG ; SONG XUE ; WANG XUE ; LYU XIN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN116790822A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>SUN JIALING</creatorcontrib><creatorcontrib>LUO XIAOMEI</creatorcontrib><creatorcontrib>ZHAO HAIFENG</creatorcontrib><creatorcontrib>XU HE</creatorcontrib><creatorcontrib>LI XINXIN</creatorcontrib><creatorcontrib>ZOU SHU</creatorcontrib><creatorcontrib>SU ZHE</creatorcontrib><creatorcontrib>HAN XIAOHONG</creatorcontrib><creatorcontrib>CHI HUI</creatorcontrib><creatorcontrib>WANG XUEHAN</creatorcontrib><creatorcontrib>FANG SHAOPENG</creatorcontrib><creatorcontrib>WANG LU</creatorcontrib><creatorcontrib>KANG XINYUAN</creatorcontrib><creatorcontrib>SHEN YUHUA</creatorcontrib><creatorcontrib>ZHOU DI</creatorcontrib><creatorcontrib>CHENG QIYUE</creatorcontrib><creatorcontrib>GAO YASONG</creatorcontrib><creatorcontrib>LI BOYUAN</creatorcontrib><creatorcontrib>HU XUESONG</creatorcontrib><creatorcontrib>SONG XUE</creatorcontrib><creatorcontrib>WANG XUE</creatorcontrib><creatorcontrib>LYU XIN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>SUN JIALING</au><au>LUO XIAOMEI</au><au>ZHAO HAIFENG</au><au>XU HE</au><au>LI XINXIN</au><au>ZOU SHU</au><au>SU ZHE</au><au>HAN XIAOHONG</au><au>CHI HUI</au><au>WANG XUEHAN</au><au>FANG SHAOPENG</au><au>WANG LU</au><au>KANG XINYUAN</au><au>SHEN YUHUA</au><au>ZHOU DI</au><au>CHENG QIYUE</au><au>GAO YASONG</au><au>LI BOYUAN</au><au>HU XUESONG</au><au>SONG XUE</au><au>WANG XUE</au><au>LYU XIN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for detecting rabies vaccine DNA (Deoxyribonucleic Acid) residue by applying digital PCR (Polymerase Chain Reaction)</title><date>2023-09-22</date><risdate>2023</risdate><abstract>The invention discloses a method for detecting rabies vaccine DNA (Deoxyribonucleic Acid) residues by applying digital PCR (Polymerase Chain Reaction). The method comprises the following steps: extracting DNA in a human rabies vaccine (Vero cell) stock solution sample; establishing a digital PCR system; preparing micro-droplets; carrying out digital PCR (Polymerase Chain Reaction) amplification; the Vero cell DNA residual copy number concentration in a human rabies vaccine sample obtained through digital PCR amplification is calculated as a national standard unit value. A primer and a probe are designed according to a GP gene sequence of the rabies virus, reaction conditions and reaction procedures are optimized, and the established digital PCR method is evaluated through specificity and repeatability tests, so that the method for detecting the human rabies vaccine DNA residues by using the digital PCR is established, and the method is good in specificity and repeatability, easy to operate and high in detecti</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | chi ; eng |
| recordid | cdi_epo_espacenet_CN116790822A |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Method for detecting rabies vaccine DNA (Deoxyribonucleic Acid) residue by applying digital PCR (Polymerase Chain Reaction) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-06T14%3A46%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=SUN%20JIALING&rft.date=2023-09-22&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN116790822A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |