Method for detecting rabies vaccine DNA (Deoxyribonucleic Acid) residue by applying digital PCR (Polymerase Chain Reaction)

The invention discloses a method for detecting rabies vaccine DNA (Deoxyribonucleic Acid) residues by applying digital PCR (Polymerase Chain Reaction). The method comprises the following steps: extracting DNA in a human rabies vaccine (Vero cell) stock solution sample; establishing a digital PCR system; preparing micro-droplets; carrying out digital PCR (Polymerase Chain Reaction) amplification; the Vero cell DNA residual copy number concentration in a human rabies vaccine sample obtained through digital PCR amplification is calculated as a national standard unit value. A primer and a probe are designed according to a GP gene sequence of the rabies virus, reaction conditions and reaction procedures are optimized, and the established digital PCR method is evaluated through specificity and repeatability tests, so that the method for detecting the human rabies vaccine DNA residues by using the digital PCR is established, and the method is good in specificity and repeatability, easy to operate and high in detecti