Infective clone vector of wild rice scotogramma virus B and construction method of infectious clone vector

The invention discloses a WrULVB infectious cloning vector and a construction method of the WrULVB infectious cloning vector, and on the basis that a WrULVB genome complete sequence is obtained by taking a wild rice diseased leaf as a material, the WrULVB infectious cloning vector is cloned to a pCassRZ binary vector containing a cauliflower mosaic virus dual-35S promoter in a multi-fragment homologous recombination manner to obtain the WrULVB infectious cloning vector. According to the invention, the virus vector pCassRZ-WrULVB which can be mediated by agrobacterium and has systematic infection capability on nicotiana benthamiana is obtained for the first time, a foundation is laid for researching virus infection, replication, movement, propagation, pathogenic mechanisms and the like through reverse genetics, and transformation of the virus into a vector tool becomes possible. 本发明公开了一种野生稻类幽影病毒B（wild rice umbra-like virus B，WrULVB）侵染性克隆载体及其构建方法，以野生稻病叶为材料获得WrULVB基因组全序列的基础上，通过多片段同源重组的方式将其克隆到含花椰菜花叶病毒双35S启动子的pCas