Phosphoenolpyruvate carboxykinase mutant and application thereof

The invention provides a phosphoenolpyruvate carboxykinase mutant and application thereof. After amino acid at the 147th site of phosphoenolpyruvate carboxykinase in bacillus microorganisms is mutated from lysine (K) to arginine (R) or histidine (H), the yield of adenosine and the yield of inosine are both increased, compared with an original strain A1, the yield of adenosine of a mutant pckAK147H engineering strain is increased to 1.8 g/L from 1.3 g/L, and the yield of inosine is increased to 1.3 g/L from 0.6 g/L. An effective means is provided for large-scale production of nucleoside, and the application prospect is wide. 本发明提供磷酸烯醇式丙酮酸羧激酶突变体及其应用。将芽孢杆菌属微生物中磷酸烯醇式丙酮酸羧激酶第147位氨基酸由赖氨酸(K)突变为精氨酸(R)或组氨酸(H)后，腺苷、肌苷产量均有提升，突变体pckAK147H工程菌株与出发菌株A1相比，腺苷产量由1.3g/L提高到1.8g/L，肌苷产量由0.6g/L提高到1.3g/L。本发明为大规模生产核苷提供有效手段，应用前景广阔。