Pyruvate kinase mutant and application thereof

The invention discloses a pyruvate kinase mutant and application thereof. After the 101 < th > amino acid of pyruvate kinase in bacillus microorganisms is mutated into lysine (K), arginine (R) or proline (P) from threonine (T), the adenosine yield is improved, and particularly, the effect is the best after threonine is mutated into lysine. Compared with the original strain A1, the pyruvate kinase mutant strain pykT101K has the advantages that the adenosine yield is increased from 1.3 g/L to 2.1 g/L, and the highest adenosine yield is increased; compared with the original strain A1, the pyruvate kinase mutant strain pykT101R has the advantage that the inosine yield is improved to 1.1 g/L from 0.6 g/L. An effective means is provided for large-scale production of nucleoside, and the application prospect is wide. 本发明公开了丙酮酸激酶突变体及其应用。将芽孢杆菌属微生物中的丙酮酸激酶第101位氨基酸由苏氨酸(T)突变为赖氨酸(K)、精氨酸(R)或脯氨酸(P)后，腺苷产量均有提升，尤其以苏氨酸突变为赖氨酸后效果最好。丙酮酸激酶突变菌株pykT101K与出发菌株A1相比，腺苷产量由1.3g/L提高到2.1g/L最高；丙酮酸激酶突变菌株pykT101R与出发菌株A1相比，肌苷产量由0.6g/L提高到1.1g/L最好。本