Method for purifying human maxillary sinus mucous membrane intrinsic layer mesenchymal stem cells and application of human maxillary sinus mucous membrane intrinsic layer mesenchymal stem cells

The invention is applicable to the technical field of biomedical treatment, and provides a purification method of human maxillary sinus mucous membrane intrinsic layer mesenchymal stem cells, which comprises the following specific steps: step 1, extracting cells; step 2, passage; and step 3, cryopreservation and resuscitation of the cells. According to the method, human-derived maxillary sinus mucous membrane intrinsic layer mesenchymal stem cells are found, extracted and cultured, and mucous membrane single sublayer in-vitro culture is different from an existing mode of performing in-vitro culture on a maxillary sinus whole layer. And the cell is synchronously subjected to osteogenic function verification, which proves that the cell can be subjected to osteogenic differentiation and has a bone tissue engineering application prospect. 本发明适用于生物医疗技术领域，提供了一种人上颌窦黏膜固有层间充质干细胞的提纯方法，包括以下具体步骤：步骤1、细胞提取；步骤2、传代；步骤3、细胞的冻存和复苏。该方法发现并提取培养了人源上颌窦黏膜固有层间充质干细胞，黏膜单一亚层体外培养，区别于目前将上颌窦全层进行体外培养的方式。并同步将该细胞进行了成骨功能验证，证明其可以成骨分化，具有骨组织工程应用前景