Genetically engineered bacterium for high-ammonia-nitrogen wastewater and construction method of genetically engineered bacterium

The invention relates to the technical field of sewage treatment and genetic engineering, in particular to a genetically engineered bacterium for high-ammonia-nitrogen wastewater and a construction method of the genetically engineered bacterium. The preservation number of the genetically engineered...

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Bibliographische Detailangaben
Hauptverfasser: DU LILI, BAO HAIHUA, XIE JIA, LIN HONGYONG, ZHANG HANYI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to the technical field of sewage treatment and genetic engineering, in particular to a genetically engineered bacterium for high-ammonia-nitrogen wastewater and a construction method of the genetically engineered bacterium. The preservation number of the genetically engineered bacterium is CCTCC NO: M 2023301, and the genetically engineered bacterium is preserved in the China Center for Type Culture Collection. Ammonia monooxygenase genes and lipase genes are loaded in genetically engineered bacteria, ammonia monooxygenase is a key enzyme in the metabolic process of nitrifying bacteria and mainly catalyzes oxidation of ammonia nitrogen to form hydroxylamine, lipase is a special ester bond hydrolase, can decompose various natural oils and fats generated by organisms, can degrade COD in sewage, and can be used as a catalyst for nitrifying bacteria. Ammonia monooxygenase and lipase are expressed through cooperation of the ammonia monooxygenase gene and the lipase gene, and ammonia nitrogen