Method for detecting RNA proportion of each component of multivalent vaccine by applying digital PCR (Polymerase Chain Reaction)

The invention relates to the technical field of detection of RNA (Ribonucleic Acid) proportions of all components in multivalent vaccines, and discloses a method for detecting the RNA proportions of all the components in the multivalent vaccines by applying digital PCR (Polymerase Chain Reaction), which comprises the following steps of: extracting total RNA in a new crown multivalent mRNA (Messenger Ribonucleic Acid) vaccine sample; establishing a digital PCR (Polymerase Chain Reaction) system by taking the total RNA as a template and using upstream and downstream primers and probes of the new coronavirus; preparing micro-droplets; carrying out digital PCR (Polymerase Chain Reaction) amplification; and calculating to obtain the RNA proportion of each component in the new crown multivalent mRNA vaccine. Primers and probes are designed according to a nuclear shell protein N gene region of the new crown virus, reaction conditions and reaction procedures are optimized, the established digital PCR method is evalua