MRNA, novel coronavirus mRNA vaccine containing mRNA and preparation method of novel coronavirus mRNA vaccine

The invention provides mRNA, a novel coronavirus mRNA vaccine containing the mRNA and a preparation method of the novel coronavirus mRNA vaccine, and relates to the technical field of nucleic acid vaccines. The mRNA comprises a sequence for encoding an antigenic polypeptide of a new coronavirus omicron mutant strain or an antigenic fragment, a variant or a derivative of the antigenic polypeptide, and the antigenic polypeptide or the antigenic fragment of the antigenic polypeptide at least comprises a receptor binding domain (RBD) fragment of a new coronavirus S protein. The invention provides the mRNA vaccine aiming at the new coronavirus Omicron mutant strain, the mRNA can express antigen protein in in-vitro cells, a high-titer new coronavirus Omicron mutant strain pseudovirus neutralizing antibody can be detected after a mouse is immunized, and the mRNA vaccine has good immunogenicity and is of great significance to prevention of the new coronavirus Omicron mutant strain virus. 本发明提供了mRNA及包含其的新冠病毒mRNA疫苗和制备方