SIEC-S cell suitable for serum-free full suspension culture, domestication method and application

The invention discloses an SIEC-S cell suitable for serum-free full suspension and a culture domestication method, and belongs to the technical field of SIEC-S cell culture.The SIEC-S cell is preserved in the China General Microbiological Culture Collection Center on December 25, 2022, the preservation number is CGMCC No.45338, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.45338. The cells are classified and named as porcine small intestinal mucosal epithelium suspension cells; the SIEC-S cells directly grow in the serum-free CD culture medium, the use amount of serum is reduced, the cost is reduced, the SIEC-S can be subjected to suspension large-scale culture on a bioreactor, and the yield of the downstream vaccine antigen content is improved. 本发明公开了一种适合无血清全悬浮的SIEC-S细胞及培养驯化方法，属于SIEC-S细胞培养技术领域，SIEC-S细胞于2022年12月25日保藏于中国微生物菌种保藏管理委员会普通微生物中心，保藏编号为CGMCCNo.45338，保藏地址为北京市朝阳区北辰西路1号院3号，分类命名为猪小肠粘膜上皮悬浮细胞；SIEC-S细胞直接在无血清的CD培养基中生长，减少了血清的使用量